Multiplex Assay:Article Title: Mutual antagonism between PRC1 condensates and SWI/SNF in chromatin regulation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cut&Run and RNAseq data This study GEO: GSE301505; NIH SRA database/ BioProject: PRJNA1284255 Experimental models: Cell lines NIH-3T3 ATCC CRL-1658 .. HCT116 ATCC CCL-247 J1 Mouse Embryonic Stem Cells (mESC) ATCC SCRC-1010 HEK293T ATCC CRL-3216 Sf9 cells Expression Systems 94-001F Oligonucleotides NEBNext® Multiplex Oligos for Illumina New England Biolabs Cat#E6440S Linear Puromycin Marker Takara Bio Cat631626 Linear Hygromycin Marker Takara Bio Cat#631625 PHC2 KO sg RNA: CCCCGGCATGACCTGTCCA Integrated DNA Technologies (IDT) N/A PHC2 L307R sgRNA: GCGCCATGAACATCAAGCTG Integrated DNA Technologies (IDT) N/A HALO PHC2 sgRNA: GACTGGCAGCTCATTCTCCA Integrated DNA Technologies (IDT) N/A mEOS3.2 ARID1A sgRNA: AGCGGGGATCATGGCCGCGC Integrated DNA Technologies (IDT) N/A PITCh gRNA: GCATCGTACGCGTACGTGTT Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side1_Biotin (Biotin/ TTT TTT TTG GTG TAG GAG GTA GAT GAG);;; Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side1_UN (TTT TTT TTG GTG TAG GAG GTA GAT GAG) Integrated DNA Technologies (IDT) N/A OligoB-BsmBI_Side1_ATTO647N or Cy3N (Phos ATCG CTC ATC TAC CTC CTA CAC CAA /ATTO647NN/) Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side2_Biotin (Phos ACTG GGT AGA GTG GTA AGT AGT GAA TTT TTT /Biotin/) Integrated DNA Technologies (IDT) N/A OligoB-BsmBI_Side2UN (/Cy3N/ TTC ACT ACT TAC CAC TCT ACC) Integrated DNA Technologies (IDT) N/A Recombinant DNA pBig1a vector Weissmann et al.84 Addgene 80611 pBig2ab vector Weissmann et al.84 Addgene 80616 pTRIPZ Dharmacon N/A pFastBac1 Thermo Fisher Scientific Cat#10359016 pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGS-CBX2 Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGSCBX2-13KRA Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGSCBX2-23KRA Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGS-CBX7 Plys et al.37 N/A pFastBac1-PHC2-TEV-HALO Niekamp et al.39 N/A pFastBac1-PHC2-L307R-TEV-HALO Niekamp et al.39 N/A pBig1a-RING1B-BMI1 Niekamp et al.39 N/A pBig1a-Brg1-ini1-baf170 This study N/A pBig1a-Brg1-K785R-ini1-baf170 This study N/A pBig1a-Brg1-D881N-ini1-baf170 This study N/A pBig2ab_baf57_dpf2_act_baf53_baf60 This study N/A pFastBac1-proa-halo-arid1a This study N/A pCRIS-PITChv2-dTAG-Puro (BRD4) Nabet et al.85 Addgene 91796 (Continued on next page) ll OPEN ACCESS Article e4 Molecular Cell 86, 1–18.e1–e15, May 7, 2026 .. Carl Zeiss Microscopy LLC Cat#474030-9000-000 Amicon Ultra-4 MW 100 kDa Sigma Cat#UFC810024 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–18.e1–e15, May 7, 2026 e5
Marker:Article Title: Mutual antagonism between PRC1 condensates and SWI/SNF in chromatin regulation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cut&Run and RNAseq data This study GEO: GSE301505; NIH SRA database/ BioProject: PRJNA1284255 Experimental models: Cell lines NIH-3T3 ATCC CRL-1658 .. HCT116 ATCC CCL-247 J1 Mouse Embryonic Stem Cells (mESC) ATCC SCRC-1010 HEK293T ATCC CRL-3216 Sf9 cells Expression Systems 94-001F Oligonucleotides NEBNext® Multiplex Oligos for Illumina New England Biolabs Cat#E6440S Linear Puromycin Marker Takara Bio Cat631626 Linear Hygromycin Marker Takara Bio Cat#631625 PHC2 KO sg RNA: CCCCGGCATGACCTGTCCA Integrated DNA Technologies (IDT) N/A PHC2 L307R sgRNA: GCGCCATGAACATCAAGCTG Integrated DNA Technologies (IDT) N/A HALO PHC2 sgRNA: GACTGGCAGCTCATTCTCCA Integrated DNA Technologies (IDT) N/A mEOS3.2 ARID1A sgRNA: AGCGGGGATCATGGCCGCGC Integrated DNA Technologies (IDT) N/A PITCh gRNA: GCATCGTACGCGTACGTGTT Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side1_Biotin (Biotin/ TTT TTT TTG GTG TAG GAG GTA GAT GAG);;; Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side1_UN (TTT TTT TTG GTG TAG GAG GTA GAT GAG) Integrated DNA Technologies (IDT) N/A OligoB-BsmBI_Side1_ATTO647N or Cy3N (Phos ATCG CTC ATC TAC CTC CTA CAC CAA /ATTO647NN/) Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side2_Biotin (Phos ACTG GGT AGA GTG GTA AGT AGT GAA TTT TTT /Biotin/) Integrated DNA Technologies (IDT) N/A OligoB-BsmBI_Side2UN (/Cy3N/ TTC ACT ACT TAC CAC TCT ACC) Integrated DNA Technologies (IDT) N/A Recombinant DNA pBig1a vector Weissmann et al.84 Addgene 80611 pBig2ab vector Weissmann et al.84 Addgene 80616 pTRIPZ Dharmacon N/A pFastBac1 Thermo Fisher Scientific Cat#10359016 pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGS-CBX2 Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGSCBX2-13KRA Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGSCBX2-23KRA Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGS-CBX7 Plys et al.37 N/A pFastBac1-PHC2-TEV-HALO Niekamp et al.39 N/A pFastBac1-PHC2-L307R-TEV-HALO Niekamp et al.39 N/A pBig1a-RING1B-BMI1 Niekamp et al.39 N/A pBig1a-Brg1-ini1-baf170 This study N/A pBig1a-Brg1-K785R-ini1-baf170 This study N/A pBig1a-Brg1-D881N-ini1-baf170 This study N/A pBig2ab_baf57_dpf2_act_baf53_baf60 This study N/A pFastBac1-proa-halo-arid1a This study N/A pCRIS-PITChv2-dTAG-Puro (BRD4) Nabet et al.85 Addgene 91796 (Continued on next page) ll OPEN ACCESS Article e4 Molecular Cell 86, 1–18.e1–e15, May 7, 2026 .. Carl Zeiss Microscopy LLC Cat#474030-9000-000 Amicon Ultra-4 MW 100 kDa Sigma Cat#UFC810024 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–18.e1–e15, May 7, 2026 e5
Recombinant:Article Title: Mutual antagonism between PRC1 condensates and SWI/SNF in chromatin regulation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cut&Run and RNAseq data This study GEO: GSE301505; NIH SRA database/ BioProject: PRJNA1284255 Experimental models: Cell lines NIH-3T3 ATCC CRL-1658 .. HCT116 ATCC CCL-247 J1 Mouse Embryonic Stem Cells (mESC) ATCC SCRC-1010 HEK293T ATCC CRL-3216 Sf9 cells Expression Systems 94-001F Oligonucleotides NEBNext® Multiplex Oligos for Illumina New England Biolabs Cat#E6440S Linear Puromycin Marker Takara Bio Cat631626 Linear Hygromycin Marker Takara Bio Cat#631625 PHC2 KO sg RNA: CCCCGGCATGACCTGTCCA Integrated DNA Technologies (IDT) N/A PHC2 L307R sgRNA: GCGCCATGAACATCAAGCTG Integrated DNA Technologies (IDT) N/A HALO PHC2 sgRNA: GACTGGCAGCTCATTCTCCA Integrated DNA Technologies (IDT) N/A mEOS3.2 ARID1A sgRNA: AGCGGGGATCATGGCCGCGC Integrated DNA Technologies (IDT) N/A PITCh gRNA: GCATCGTACGCGTACGTGTT Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side1_Biotin (Biotin/ TTT TTT TTG GTG TAG GAG GTA GAT GAG);;; Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side1_UN (TTT TTT TTG GTG TAG GAG GTA GAT GAG) Integrated DNA Technologies (IDT) N/A OligoB-BsmBI_Side1_ATTO647N or Cy3N (Phos ATCG CTC ATC TAC CTC CTA CAC CAA /ATTO647NN/) Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side2_Biotin (Phos ACTG GGT AGA GTG GTA AGT AGT GAA TTT TTT /Biotin/) Integrated DNA Technologies (IDT) N/A OligoB-BsmBI_Side2UN (/Cy3N/ TTC ACT ACT TAC CAC TCT ACC) Integrated DNA Technologies (IDT) N/A Recombinant DNA pBig1a vector Weissmann et al.84 Addgene 80611 pBig2ab vector Weissmann et al.84 Addgene 80616 pTRIPZ Dharmacon N/A pFastBac1 Thermo Fisher Scientific Cat#10359016 pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGS-CBX2 Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGSCBX2-13KRA Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGSCBX2-23KRA Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGS-CBX7 Plys et al.37 N/A pFastBac1-PHC2-TEV-HALO Niekamp et al.39 N/A pFastBac1-PHC2-L307R-TEV-HALO Niekamp et al.39 N/A pBig1a-RING1B-BMI1 Niekamp et al.39 N/A pBig1a-Brg1-ini1-baf170 This study N/A pBig1a-Brg1-K785R-ini1-baf170 This study N/A pBig1a-Brg1-D881N-ini1-baf170 This study N/A pBig2ab_baf57_dpf2_act_baf53_baf60 This study N/A pFastBac1-proa-halo-arid1a This study N/A pCRIS-PITChv2-dTAG-Puro (BRD4) Nabet et al.85 Addgene 91796 (Continued on next page) ll OPEN ACCESS Article e4 Molecular Cell 86, 1–18.e1–e15, May 7, 2026 .. Carl Zeiss Microscopy LLC Cat#474030-9000-000 Amicon Ultra-4 MW 100 kDa Sigma Cat#UFC810024 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–18.e1–e15, May 7, 2026 e5
Plasmid Preparation:Article Title: Mutual antagonism between PRC1 condensates and SWI/SNF in chromatin regulation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Cut&Run and RNAseq data This study GEO: GSE301505; NIH SRA database/ BioProject: PRJNA1284255 Experimental models: Cell lines NIH-3T3 ATCC CRL-1658 .. HCT116 ATCC CCL-247 J1 Mouse Embryonic Stem Cells (mESC) ATCC SCRC-1010 HEK293T ATCC CRL-3216 Sf9 cells Expression Systems 94-001F Oligonucleotides NEBNext® Multiplex Oligos for Illumina New England Biolabs Cat#E6440S Linear Puromycin Marker Takara Bio Cat631626 Linear Hygromycin Marker Takara Bio Cat#631625 PHC2 KO sg RNA: CCCCGGCATGACCTGTCCA Integrated DNA Technologies (IDT) N/A PHC2 L307R sgRNA: GCGCCATGAACATCAAGCTG Integrated DNA Technologies (IDT) N/A HALO PHC2 sgRNA: GACTGGCAGCTCATTCTCCA Integrated DNA Technologies (IDT) N/A mEOS3.2 ARID1A sgRNA: AGCGGGGATCATGGCCGCGC Integrated DNA Technologies (IDT) N/A PITCh gRNA: GCATCGTACGCGTACGTGTT Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side1_Biotin (Biotin/ TTT TTT TTG GTG TAG GAG GTA GAT GAG);;; Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side1_UN (TTT TTT TTG GTG TAG GAG GTA GAT GAG) Integrated DNA Technologies (IDT) N/A OligoB-BsmBI_Side1_ATTO647N or Cy3N (Phos ATCG CTC ATC TAC CTC CTA CAC CAA /ATTO647NN/) Integrated DNA Technologies (IDT) N/A OligoA-BsmBI_Side2_Biotin (Phos ACTG GGT AGA GTG GTA AGT AGT GAA TTT TTT /Biotin/) Integrated DNA Technologies (IDT) N/A OligoB-BsmBI_Side2UN (/Cy3N/ TTC ACT ACT TAC CAC TCT ACC) Integrated DNA Technologies (IDT) N/A Recombinant DNA pBig1a vector Weissmann et al.84 Addgene 80611 pBig2ab vector Weissmann et al.84 Addgene 80616 pTRIPZ Dharmacon N/A pFastBac1 Thermo Fisher Scientific Cat#10359016 pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGS-CBX2 Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGSCBX2-13KRA Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGSCBX2-23KRA Plys et al.37 N/A pFastBac1-Flag-GSAAAGS-mGFP-GSAAAGS-CBX7 Plys et al.37 N/A pFastBac1-PHC2-TEV-HALO Niekamp et al.39 N/A pFastBac1-PHC2-L307R-TEV-HALO Niekamp et al.39 N/A pBig1a-RING1B-BMI1 Niekamp et al.39 N/A pBig1a-Brg1-ini1-baf170 This study N/A pBig1a-Brg1-K785R-ini1-baf170 This study N/A pBig1a-Brg1-D881N-ini1-baf170 This study N/A pBig2ab_baf57_dpf2_act_baf53_baf60 This study N/A pFastBac1-proa-halo-arid1a This study N/A pCRIS-PITChv2-dTAG-Puro (BRD4) Nabet et al.85 Addgene 91796 (Continued on next page) ll OPEN ACCESS Article e4 Molecular Cell 86, 1–18.e1–e15, May 7, 2026 .. Carl Zeiss Microscopy LLC Cat#474030-9000-000 Amicon Ultra-4 MW 100 kDa Sigma Cat#UFC810024 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–18.e1–e15, May 7, 2026 e5
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